- IH-SR Core System for Isolated Small Rodent Heart
The IH-SR Langendorff Core System is the starting point for all isolated perfused heart experiments on small rodents such as mouse, rat and guinea pig in the Langendorff retrograde perfusion mode.
The core Langendorff system can be easily upgraded to a working heart-ready (ejecting heart) configuration any time later.
Because of its larger heart chamber, this system is well-suited for applications that require ECG and MAP recordings in addition to LVP and pacing. The fully closing heart chamber allows for precise temperature control, making it the optimal choice for mouse heart perfusion.
- IH-SR Core System Options
Additions to the IH-SR Core System for species-specific Langendorff Only or Langendorff Working Heart-Ready configurations.
Options must be selected for a functional unit.
- Working Heart Upgrade for IH-SR Core System
Upgrade an IH-SR Langendorff configuration to an IH-SR Working Heart (according to Neely) System.
- Fully-ejecting working heart model for physiological cardiovascular studies in small rodents.
- Allows rapid and easy switching between Working Heart (Ejecting Heart) and Langendorff modes.
- Measurement of pO2, pH, pCO2 for All Isolated Organ Systems
The Universal Perfusion Solution Monitor permits precise continuous or discontinuous measurement in liquid media or perfusate of these three key parameters: pO2, pH and pCO2
- Flow Measurement Options for IH-SR and IH-5 in Langendorff and Working Heart Modes
Flow measurement can be accomplished in a functional IH system using two methods:
- Indirect measurement by controlling the pump sped of a roller pump (SCP controller)
- Direct measurement with ultrasound transit time technique (TTFM-2 module)
Required components are included in the IH-SR or IH-5 Core Systems and the selected IH-SR or IH-5Core Systems Options.
- Left Ventricular Pressure (LVP) Measurement Option for IH-SR and IH-5 Working Heart
Easily allows introduction of a tip pressure catheter directly into the left ventricle via the adapter port and the aorta, rather than via apical puncture.
- Increased Preload Pressure Option for IH-SR Working Heart
Choose this option when you need to create left atrial preload pressures that are greater than 11 mmHg.
- Cannulating Options for IH-SR and IH-5 (for Effluate Collection)
Cannulating options for effluate collection from an IH-SR or IH-5 functional system for metabolic studies, sampling the effluate for analysis, or for continuous pO2, pH, pCO2 measurements. Preparation dish and collection funnel available for mouse heart.
- Single-Lead ECG and MAP Measurement for IH-SR and IH-5 Langendorff or Working Heart
For single-lead ECG and MAP measurements on functional IH-SR or IH-5 Langendorff or working heart systems. ECG and MAP are recorded to be analyzed together.
The Monophasic Action Potential (MAP) electrodes work based on the principle of Franz et al whereby firm, stable contact of the MAP electrode results in a high-quality MAP. MAP recording has been utilized in cardiac physiology for decades as it allows regional assessment of the electrophysiological state of the intact heart.
For additional options available for the IH-5 system, please see Multi-Lead ECG and MAP Measurement Options for IH-5 Langendorff or Working Heart.
