- IPL-1 Core Isolated Perfused Lung System for Mouse
The IPL-1 is an isolated organ perfusion system specifically engineered for the fragile mouse lung. The basic system can easily be upgraded to add capability modules for virtually any ex vivo pulmonary experiment.
Due to the fragile nature of the mouse lung, its suspension from the tracheal and vascular cannulae is difficult to accomplish without the rapid occurrence of edema. In collaboration with experts in pulmonary physiology, a design was developed which allows for ventilation and perfusion with the lungs remaining in situ at a slight incline in the open thorax.. This novel approach simplifies cannulation of the pulmonary artery and left atrium of the heart and dramatically reduces edema formation.
The isolated mouse lung can be ventilated under positive pressure and under subatmospheric pressure to mimi the in vivo situation as closely as possible. There are bigger differences in vascular flows seen between positive and negative ventilation. Depending on the study, a ventilated lung or a physiologic breathing lung can be simulated.
Please note: For a functional unit, the core system requires the addition of core system options for ventilation.
- Deoxygenation Unit for IPL-1 (DEXOY150-1) or IPL-2 (DEXOY150-2)
For deoxygenation of blood or buffers containing proteins (e.g. albumin) or erythrocytes
- pH Control Unit for IPL-1 (pHC-1)
Delivers CO2 to maintain pH when system is not deoxygenated with N2/CO2 gas mixture
- Measurement of pO2, pH, pCO2 for All Isolated Organ Systems
The Universal Perfusion Solution Monitor permits precise continuous or discontinuous measurement in liquid media or perfusate of these three key parameters: pO2, pH and pCO2.
It can be used in any isolated organ system: heart, lung, liver, kidney or other organs. It also can be combined with any self-built perfusion system by adding a suitable PLUGSYS housing for the listed modules.
- Venous Pressure Measurement for all IPL Systems (VPM)
Venous pressure transducer and amplifier for venous pressure measurement in an isolated perfused lung system. Amplifier module requires suitable PLUGSYS housing.
- Drug Addition Options for All Isolated Organ Systems
These options for drug addition can be added to any isolated organ system where flow is measured or calculated and a drug must be added in a certain ratio.
There are two options:
- Perfusion flow-controlled drug addition with syringe pump: allows a drug addition proportional to the measured flow in order to maintain a stable drug concentration at any (constant pressure) flow rate. Requires HSE software to read and calculate the flow to control the syringe pump flow.
- Perfusion drug addition with syringe pump: allows drug addition at a set flow rate.
- Temperature Measurement Addition for All Isolated Organ Systems
Measure perfusate temperature in any isolated perfused organ system in one of two ways:
- Using the PLUGSYS Thermocouple Amplifier Module for continuous recording of temperature
- Using a handlheld thermometer showing digital display, no continuous recording possibility
- Addition for Filtration of Recirculated Perfusate for All Isolated Organ Systems
Includes:
- In-line Holder for disc particle filter, Diameter = 47 mm
- Polypropylene Particle Filter, 45 µm, Diameter = 47 mm (pack of 100)
